Synaptophysin Monoclonal Antibody

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Catalogue Numbers: MB66637-50, MB66637-100

Sizes: 50µl, 100µl

Swiss-Prot: P08247

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that is expressed in neuroendocrine cells and neoplasms. Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore. Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases. Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex.

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of Synaptophysin protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Synaptophysin expression in rat brain (A), rat hippocampus (B), Hela (C), NIH3T3 (D) whole cell lysates., Immunohistochemical analysis of Synaptophysin staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.140). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 38 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Synaptophysin; Major synaptic vesicle protein p38

Immunogen: Purified recombinant fragment of SYP expressed in E. Coli.

Conjugate: Unconjugated

Modification: Unmodified