EAR2 (S40) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1925-50, BS1925-100

Sizes: 50µl, 100µl

Swiss-Prot: P10588

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: EAR2 (v-erb-a avian erythroblastic leukemia viral oncogene homolog-like 2) protein binds a cis enhancer element (TGACCT motif) upstream of the rennin gene and represses renin gene transcription. EAR2 also contributes to gonadotropin-dependent derepression of LHR promoter activity in granulose cells. EAR2 mRNA is abundant in the liver, and EAR2 protein localizes to the nucleus of As4.1 cells.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: EAR2 (S40) polyclonal antibody detects endogenous levels of EAR2 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of EAR2 (S40) pAb at 1:500 dilution
Lane 1: PC12 whole cell lysate (40ug)
Lane 2: AML-12 whole cell lysate (40ug)
Lane 3: HEK293T whole cell lysate (40ug)
Lane 4: HepG2 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of EAR2 (S40) pAb in paraffin-embedded human stomach carcinoma tissue at 1:50.showing Nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 42 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Probable glutamyl-tRNA synthetase; mitochondrial; Glutamate--tRNA ligase; GluRS; EARS2; KIAA1970

Immunogen: Synthetic peptide, corresponding to amino acids 21-70 of Human EAR2.

Conjugate: Unconjugated

Modification: Unmodified