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ClickTech Library Kit full-length mRNA, 1 kit, cardboardClickTech Library Kit full length mRNA, Density (D) ~1 g cm, Storage temp. 20 C, UN Nr. 1760 The ClickTech Library Kit can be used to sequence the whole mRNA or exome of cells for genetic diagnosis. Here, an azide modified cDNA is created from any RNA pool, which can then be highly specifically clicked with an alkyne modified adapter sequence. The 3' adapter clicked cDNA can be amplified via PCR and used for further experiments. The kit contains: dNTP
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ClickTech Library Kit full-length mRNA, Density (D) ~1 g/cm³, Storage temp. -20 °C, UN-Nr. 1760

The ClickTech Library Kit can be used to sequence the whole mRNA or exome of cells for genetic diagnosis. Here, an azide-modified cDNA is created from any RNA pool, which can then be highly specifically clicked with an alkyne-modified adapter sequence. The 3'-adapter-clicked cDNA can be amplified via PCR and used for further experiments.

The kit contains:
  • dNTP Mix (10 mM)
  • LFRS Primer RT (100 µM)
  • 5x Reverse Transcription Buffer
  • DTT (100 mM)
  • Reverse Transcriptase (200 U/µL)
  • 10x RNase H Buffer
  • RNase H (5 U/µL)
  • RNase A (10 mg/mL)
  • 5x Azide Elongation Buffer
  • 3’-N3-ddGTP (10 mM)
  • Azide Elongase (20 U/µL)
  • 5x Activator2
  • Reactor XS
  • Alkyne Adapter (100 µM)
  • 2x baseclick PCR Master Mix
  • LFRS Primer Forward (10 µM)
  • LFRS Primer Reverse (10 µM)

ClickTech Library Kit full-length mRNA, 1 kit, cardboard

Item no : 33511875250
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US$ 588.58
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Min. order: 1piece

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