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home > ROTIPHORESE® 10x TBE Buffer, 10x conc. buffer concentrate, 1 l, glass > ROTIPHORESE® 10x TBE Buffer, 10x conc. buffer concentrate, 1 l, glass

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ROTIPHORESE® 10x TBE Buffer, 10x conc. buffer concentrate, 1 l, glassROTIPHORESE 10x TBE Buffer, 10x conc. buffer concentrate, Density (D) 1,068 g cm, Boiling point (bp) >100 C, Storage temp. +15 to +25 C The TBE buffer is optimally suited as a gel and running buffer system. Mechanism TBE buffer has a higher buffer capacity than TAE, but also leads to stronger heating of the gels. A slightly lower voltage should therefore be selected when gelling. DNA migrates in the TBE gel about half as fast as in TAE buffered gels.

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ROTIPHORESE® 10x TBE Buffer, 10x conc. buffer concentrate, Density (D) 1,068 g/cm³, Boiling point (bp) >100 °C, Storage temp. +15 to +25 °C

The TBE buffer is optimally suited as a gel and running buffer system.

Mechanism

TBE buffer has a higher buffer capacity than TAE, but also leads to stronger heating of the gels. A slightly lower voltage should therefore be selected when gelling. DNA migrates in the TBE gel about half as fast as in TAE-buffered gels.ROTIPHORESE^®TBE buffer has a high buffer capacity and is therefore ideally suited as a gel and running buffer system for sequencing gels.

ROTIPHORESE^®10x TBE buffer contains 1,0 M Tris-Borate pH 8,3 and 20 mM EDTA in distilled, deionised water; pH 8,3. Suitable for manufacturing sequencing gels with ROTIPHORESE^®Gel 40 and as a running buffer.

Directions for use

ROTIPHORESE^®TBE buffer is usually used as 1x or 0.5-fold concentrated solution. TBE buffer is particularly suitable for the separation of small DNA fragments in the gel (up to 3 kb). TBE buffer is not suitable for use of gels for gel extraction of DNA.

Technical Documentation (PDF)

ROTIPHORESE® 10x TBE Buffer, 10x conc. buffer concentrate, 1 l, glass

Name: ROTIPHORESE® 10x TBE Buffer, 10x conc. buffer concentrate, 1 l, glass
Brand: nj-cca.org
SKU: 26943255763
Price: 37.55 USD
Availability: InStock
Rating: 4.4914000000000005 (844 reviews)