Loading bufer with the fluorescent dye SYBR^® Green I for staining of double stranded nucleic acid in agarose and polyacrylamide gels.

ROTI^®Load DNAstain SYBR^® Green is easy to use and reduces significantly the time required for gel analysis. The contained fluorescent dye SYBR^® Green I detects up to 0.01 ng per band, and is, therefore, 20 times more sensitive than ethidium bromide. DNA integrity is not influenced by SYBR^® Green I; hence, gel eluted DNA may directly be applied to standard down-stream applications, like, e.g., ligation, PCR, transformation, transfection etc. without interfering effects.SYBR^® Green I bounds to the minor groove of DNA and intercalates into the DNA. It can be excited by UV- and blue light, making it fully compatible with standard UV-transilluminators as well as with modern blue-light illumination (see also ROTIPHORESE^®PROfessional runVIEW, Art. No. 4849.1).When excited, nucleic acid-bound SYBR^® Green I emits a brightly coloured green fluorescence (521 nm) that may be documented by all usual broadband ethidium bromide- or SYBR^® Green foto filters.The figure depicts 50 ng DNA per band, or 75 ng (1000 bp and 2.5 kb), respectively. Each lane has been loaded and concurrently stained using 1 µl ROTI^®Load DNAstain SYBR^® Green.

Excitation maximum (bound to DNA): 254 nm and 495 nmEmission maximum (bound to DNA): 521 nm

In order to cover the main size range needed in gel electrophoresis, ROTI^®Load DNAstain 1 Green contains bromophenol blue and xylene cyanol.

Glycerin is the standard reagent for increasing sample density.

Recommended for staining of dsDNA- and dsRNA-fragments of over 500 bp.